RESUMO
Este artículo descubre, comenta y revisa la tesis doctoral defendida por el doctor Manuel Pascual Alonso en 1907. En ella se expone el estado material e instrumental, agentes médicos implicados y las prácticas quirúrgicas de alcance en las casas de socorro, verdaderos antecedentes de los servicios médicos de urgencia actuales. Tal tesispionera es un hallazgo relevante e iluminativo para la medicina española de urgencias y emergencias y que pone de manifiesto, a partir de este estudio retrospectivo, una valiosa y multisecular tradición médica de urgencias en España desconsiderada hasta ahora (AU)
This review and commentary of the doctoral thesis defended by Dr. Manuel Pascual Alonso in 1907, summarizes this pioneering authors account of the material and instrumental conditions in the emergency care facilities known as casas de socorro (first-aid or rescue facilities), the true forerunners of todays emergency health services. Dr. Pascual describes physician staffing and surgical practices in these centers. The thesis sheds light on the history of Spanish emergency medicine, revealing that Spain has a worthy, centuries-long tradition of emergency medical practice that has been underappreciated until now (AU)
Assuntos
Humanos , Medicina de Emergência/história , Medicina de Desastres/história , História da Medicina , Dissertações Acadêmicas como Assunto , Socorro em Desastres/históriaRESUMO
Macrophage infectivity potentiators are membrane proteins described as virulence factors in bacterial intracellular parasites, such as Legionella and Chlamydia. These factors share amino acid homology to eukaryotic peptidyl-prolyl cis-trans isomerases that are inhibited by FK506, an inhibitor of signal transduction in mammalian cells with potent immunosuppressor activity. We report here the characterization of a protein released into the culture medium by the infective stage of the protozoan intracellular parasite Trypanosoma cruzi. The protein possesses a peptidyl-prolyl cis-trans isomerase activity that is inhibited by FK506 and its non-immunosuppressing derivative L-685,818. The corresponding gene presents sequence homology with bacterial macrophage infectivity potentiators. The addition of the protein, produced heterologously in Escherichia coli, to cultures of trypomastigotes and simian epithelial or HeLa cells enhances invasion of the mammalian cells by the parasites. Antibodies raised in mice against the T.cruzi isomerase greatly reduce infectivity. A similar reduction of infectivity is obtained by addition to the cultures of FK506 and L-685,818. We concluded that the T.cruzi isomerase is involved in cell invasion.
Assuntos
Isomerases de Aminoácido/fisiologia , Proteínas de Transporte/fisiologia , Imunofilinas , Trypanosoma cruzi/enzimologia , Isomerases de Aminoácido/antagonistas & inibidores , Isomerases de Aminoácido/genética , Sequência de Aminoácidos , Animais , Anticorpos Antiprotozoários , Proteínas de Bactérias/genética , Sequência de Bases , Proteínas de Transporte/antagonistas & inibidores , Proteínas de Transporte/genética , Linhagem Celular , Clonagem Molecular , Primers do DNA/genética , DNA de Protozoário/genética , Escherichia coli/genética , Proteínas de Escherichia coli , Genes de Protozoários , Células HeLa , Humanos , Cinética , Proteínas de Membrana/genética , Camundongos , Dados de Sequência Molecular , Peptidilprolil Isomerase , Tacrolimo/farmacologia , Trypanosoma cruzi/genética , Trypanosoma cruzi/patogenicidadeRESUMO
The comparison and characterization of different populations of Trichinella has become possible through the study of their complex polypeptide profiles. Techniques such as polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF) provide a high capacity of resolution. We used both techniques to compare proteinograms of different Trichinella isolates, in order to characterize an isolate detected in a wild boar captured in Güejar-Sierra (Province of Granada, Spain).
